Introduction: People’s facial appearance can play an important role in their social success. On the other hand, facial appearance-related problems have an important effect on an individual’s quality of life. The aim of this research was to determine the relationship between dentofacial malformations and self-esteem and Self-Concept among male and female students of Isfahan Universities.Materials and Methods: In this cross-sectional research, 159 students, 18-25 years of age, were randomly selected from Isfahan Universities. The subjects consisted of 84 male and 75 female students, selected using easy sampling method. The subjects filled two Rosenberg self-esteem and Rogers Self-Concept questionnaires (A: the real self; B: the ideal self) and were examined in relation to dentofacial deformities. Data was recorded in two groups of dentofacial deformity and free of dentofacial deformity. T-test, ANOVA and Pearson’s correlation coefficient were used for data analysis (a=0.05).Results: The results did not show any relationship between self-esteem (p value=0.342), selfconcept A (p value=0.581) and Self-Concept B (p value=0.720) in the two groups. There was no relationship between deformity type and self-esteem (p value=0.71), Self-Concept A (p value=0.49) and Self-Concept B (p value=0.337). In addition, there was no relationship between sex and selfesteem (p value=0.237), Self-Concept A (p value=0.416) and Self-Concept B (p value=0.081). However, there was a direct relationship between self-esteem and Self-Concept A (r=-0.31).Conclusion: Under the limitations of this research, there was no relationship between dentofacial deformities and self-esteem and Self-Concept.